FIG. 1.

(A) Wild-type G. zeae MAT locus; (B) MAT locus deletion vector, pGzMAT-H, shown in the linear form that would result from digestion with SalI; (C) MAT locus in which the 9.6-kb region with the four MAT genes has been deleted and replaced by a 5.1-kb fragment carrying the hygB and pCR XL TOPO portions of pGzMAT-H; (D) MAT locus complementation vector pGzMAT-Gen. Numbered arrows indicate relative positions and orientations of PCR primers used in this study. The black bars in panels A and C and the white bars in panels A and D indicate the regions of DNA used to prepare the hybridization probe for Southern analysis of transformants carrying pGzMAT-Gen.