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Purification of AptA produced by E. coli BL21
| Purification step | Total protein (mg) | Sp act (U/mg) | Yield (%) | Fold purifi- cation |
|---|---|---|---|---|
| Crude extract | 430 | 9.6 | 100 | 1 |
| 0.8% Polyethyleneimine | 134 | 18.5 | 60 | 1.9 |
| Ammonium sulfate (20∼60%) | 78.4 | 27.9 | 53 | 2.9 |
| Butyl-Sepharose FF | 30.1 | 52.2 | 38 | 5.4 |
| Sephacryl S-200 FF | 11.8 | 77.2 | 22 | 8.0 |
