Fig. 5.

Analysis of PTS2 processing ability of atcml3 and atdeg15 mutant lines. Only the full-length AtDEG15 processes pre-thiolase to the mature thiolase in planta. A and B The atcml3 mutant line (pst16586, RIKEN)—unlike the atdeg15 mutant line (SALK007184)—is not impaired in processing of pre-gMDH to the mature gMDH (A) or pre-thiolase to the mature thiolase (B) as shown by western blot analysis of protein extracts using antibodies directed against gMDH or thiolase. C Analysis of several AtDEG15 variants shows that only full-length AtDEG15 processes pre-thiolase to mature thiolase as shown by western blot analysis with α-thiolase antibodies. Loss of the N-terminal CML binding-domain, loss of the C-terminus, loss of N- and C-terminus or of the loop within the protease domain all abolish processing. MM, prestained protein molecular weight markers (red, 80 kDa; blue: 58, 46, 30 kDa; green 23 kDa; BioLabs)