FIG. 3.

Conservation of ORFs in the genomic region harboring 4bSF7 and 4bSF18 between ECII and other serotype 4b strains. DNA probes were generated with the primers listed in Table 2 and used in hybridizations as described in Materials and Methods. + and − indicate the presence and absence of a hybridization signal, respectively. L. monocytogenes strains of serotype 4d yielded results identical to those of other 4b strains. ORFs which lacked detectable homology in ECII strains (on the basis of the Southern blot data) are indicated in black, and serotype 4b-specific ORFs conserved in ECII strains are indicated in gray. The putative ORFs 1373 and 1378 (111 and 90 bp, respectively) were too short for adequate probe construction, and their conservation in ECII strains has not been determined. Arrows indicate the direction of transcription. IGS, intergenic regions. Note that inlA (ORFs 1358), inlB (ORF 1355), and ORF 1376 are much longer than the others, as detailed in Table 3, and are not drawn to scale.