Figure 2.
hMSC expansion and differentiation on mineral coatings. (A) hMSC expansion on mineral coatings after 8 and 16 days in growth medium, as measured by total DNA quantification. (B) hMSC expansion and (C) ALP activity on mineral coatings after 8 days in growth (GM) or OS medium (GM-OS). (D) Correlation between ALP activity and cell expansion ratio. ALP activity of hMSC after 16 days of culture (normalized to total DNA) was plotted versus cell expansion ratio (total DNA at 16 days divided by total DNA at 8 days). Statistical significance between hMSC behaviors on the same mineral coatings (denoted by %, Student’s t-test) or on different mineral coatings respect to 5 – 2 (denoted by *,#, Dunnett t-test) at different time points (A) or in medium conditions (B and C) was set at p<0.05. Data represents mean ± SD (n = 4).
