Figure 3.

Separation of RKIP-interacting proteins with anti-flag M2 magnetic beads and 1D SDS-PAGE. A. After the affinity magnetic bead based purification, with anti-flag M2 magnetic beads, of total protein from the cells, the protein sample was pre-separated by 1D-SDS-PAGE using a 10% acrylamide gel. The clear channel proteins (n = 14) were separated in the experimental group. B. Western blot analysis to test RKIP-3xFLAG expression in different groups of affinity purified cells. After the affinity magnetic bead based purification, with anti-flag M2 magnetic beads, of total protein from the cells, RKIP-3xFLAG expression was detected in only the RKIP-3xFLAG group, whereas no RKIP-3xFLAG expression was detected in the 3xFLAG and blank groups.