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. 2013 Sep 4;64(16):4863–4875. doi: 10.1093/jxb/ert272

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© The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Expression of CTR1p:OsCTR2 complements ctr1-1. (A, B) Phenotype (A) and hypocotyl measurements (B) of etiolated seedlings. L, transformation line. (C, D) Expression of the CTR1p:OSCTR2 transgene in ctr1-1 confirmed by RT-PCR (C) and quantified (D). (E, F) Phenotype of light-grown seedlings (E) and rosettes (F) of ctr1-1 and ctr1-1 expressing CTR1p:OsCTR2. (G) qRT-PCR analysis of mRNA level of ERF1. (H, I) Expression of CTR1 in Arabidopsis (H) and of OsCTR2 but not OsCTR3 in rice (I) is ethylene inducible. (J, K) Yeast two-hybrid assay (J) and kinetics of β-galactosidase activity (K) for the interaction between ETR1/ERS1 and the OsCTR2 N terminus. Data are means ±SD of three independent experiments performed in triplicate. pBTM and pGADT7 are the vectors for the yeast two-hybrid assay.