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. 2013 Sep 4;64(16):4895–4905. doi: 10.1093/jxb/ert278

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© The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — as2-5D and iso-2D leaves contain different expression levels of polarity genes. (A, B) qRT-PCR to analyse mature leaves for AS2 (A) and FIL (B) expression levels in Col-0, as2-5D, and iso-2D. (C) qRT-PCR analyses of leaf polarity controlling genes KAN1,KAN2, ARF3, and YAB5 in Col-0, as2-5D, and iso-2D mature leaves. The qRT-PCR results were normalized to that produced by the primers at ACTIN, and the value of the wild type was arbitrarily fixed at 1.0. Bars show s.e. * and **, significant difference by t-test (*, P <0.05; **, P <0.01). (D–F) In situ hybridization to analyse developing leaf primordia for FIL expression patterns using an antisense FIL probe on transverse sections of Col-0 (D), as2-5D (E), and iso-2D (F) shoot apices. (G) The FIL sense probe control.