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. 2013 Sep 4;64(16):4895–4905. doi: 10.1093/jxb/ert278

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© The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — iso-2D leaves carry altered levels of histone modification markers H3K27me3 and H3K4me3 at the AS2 locus. (A) Diagram of the AS2 gene with primer positions (I and II) in ChIP analysis. (B, C) Compared with those in Col-0 and as2-5D, the H3K27me3 level in the iso-2D mutant was dramatically reduced (B) whereas the H3K4me3 level was elevated (C) at the AS2 locus. The ChIP results were normalized to those produced by the primers at PI (B) and ACTIN (C). Values of the wild type were arbitrarily fixed at 1.0. Bars show s.e. **, significant difference by t-test (P <0.01).