FIG. 6.

Expression of cytokines in various organs of mice. Inbred BALB/c mice were inoculated with 10¹⁰ spores of Bacillus strain PY79 (A) or Biosubtyl^NT (B). At different times, cytokine expression in various organs and tissues of the animals was detected with total RNAs by using RT-PCR. A graph showing the results of densitometric analyses (typical of the entire group) of RT-PCR data for one mouse is shown in each case; the percent expression represents the relative abundance of each cytokine compared to the abundance of β-actin. The dotted lines indicate the relative abundance of each cytokine at zero time (100%). No expression was detected in naïve, nonimmunized mice at any time (data not shown).