TABLE 1.
Bacteriocin production by probiotic strainsa
Indicator organismb | Enterogermina (B. clausii) | BiosubtylDL (B. cereus) | Bactisubtil (B. cereus) | BiosubtylNT (B. pumilus) | Subtyl (B. cereus) |
---|---|---|---|---|---|
L. innocua | −c | − | − | − | − |
P. aeruginosa NCTC 12903 | − | − | − | − | − |
B. megaterium 899 (= BGSC 7A1) | +/− | − | − | +++ | − |
B. cereus T (= BGSC 6A1) | − | − | − | +/− | − |
B. licheniformis 9945A (= BGSC 5A2) | − | − | − | − | − |
B. cereus var. vietnami (Subtyl) | − | − | − | − | +/− |
B. cereus (BiosubtylDL) | − | − | − | +/− | − |
B. pumilus (BiosubtylNT) | − | − | − | − | +/− |
B. clausii DSM 8716 | − | − | − | − | +/− |
B. sphaericus BGSC 13A5 | − | − | − | ++ | +(+) |
B. sphaericus BGSC 13A6 | − | − | − | +(+) | + |
+, clear halo of growth inhibition at least one time (results were determined after 5, 8, 24, and 48 h of incubation); +++, ++, and (++), different degrees of inhibitory activity, as assessed by decreased diameter of inhibition zone, respectively; −, no inhibition; +/−, clear reduction in growth but not complete inhibition.
All the strains were assayed two to four times.
After proteinase K treatment Enterogermina showed clear inhibitory activity with L. innocua but no activity with B. megaterium and C. perfringens. No other producers or indicators were tested under these conditions.