. 2004 Apr;70(4):2161–2171. doi: 10.1128/AEM.70.4.2161-2171.2004

TABLE 2.

Potential virulence traits of commercial strains

Strain or product	Taxon^a	Hemolysis^b	Lecithinase^c	Hbl complex genes^d				Oxoid kit test index^e	Nhe complex genes^d			Tecra kit test index^f	cytK^d	bceT^d
Strain or product	Taxon^a	Hemolysis^b	Lecithinase^c	hblA	hblB	hblC	hblD	Oxoid kit test index^e	nheA	nheB	nheC	Tecra kit test index^f	cytK^d	bceT^d
PY79	B. subtilis	γ	−	−	−	−	−	0	−	−	−	2	−	−
Subtyl	B. cereus var. vietnami	β	+	−	−	−	−	0	+	+	+	5	−	−
Biosubtyl^DL	B. cereus	α	+	+	−	+	+	128	+	+	+	4	−	+
Bactisubtil	B. cereus	β	+	+	−	+	+	256	−	+	+	2	−	+

Species were assigned by 16S rRNA analysis (13, 17). Subtyl has also been referred to as Bacillus vietnami pending approval of a new species (17).

α, brownish zone around colonies; β, complete hemolysis, with clear zone around each colony; γ, no change. In a previous study it was reported that Biosubtyl^DL produces beta hemolysis (17).

+, blue precipitation of hydrolyzed lecithin around peacock blue colonies (typical of B. cereus); −, no change.

+, a PCR product of the expected size was observed; −, no PCR product was observed.

For the Oxoid test, the indices corresponded to the last supernatant dilution (in twofold serial dilutions) for which enterotoxin remained detectable. Strains with an index of 0 were considered negative, and the sensitivity of the test is 2 ng/ml.

For the Tecra test, indices from 1 to 5 corresponded to the intensity of coloration. According to the manufacturer's instructions, strains with an index of <3 are considered negative, and the sensitivity of the test is 1 ng/ml.