Table 1.
Identification of 32 proteins associated with dormancy release treated with GA4 in Japanese apricot
| Spot no.a | Protein (taxonomy) | Accession no.b | NPc | Theoretical MW/pI | Experimental MW/pI | Scored | SC (%)e | Average fold changef |
|---|---|---|---|---|---|---|---|---|
| Stress and defence | ||||||||
| 1 | Mal d1 homologue (Prunus armeniaca) | gi|2460186 | 4 | 17.60/5.79 | 17.54/6.43 | 432 | 28 | –3.39 |
| 2 | Heat-shock protein 60 (Prunus persica) | ppa003391 | 3 | 61.50/5.62 | 59.02/5.36 | 97 | 5 | +2.92 |
| 3 | Heat-shock protein 60 (Prunus persica s) | ppa004110 | 4 | 56.32/5.19 | 60.45/5.51 | 56 | 11 | +2.59 |
| 5 | class IV chitinase (Corylus heterophylla) | gi|344190188 | 2 | 30.03/5.21 | 27.21/4.24 | 116 | 6 | +20.33 |
| 6 | Pathogenesis-related thaumatin superfamily protein (Prunus persica) | ppa010479 | 5 | 26.94/4.83 | 37.55/4.50 | 125 | 33 | +7.00 |
| Energy metabolism | ||||||||
| 8 | Alcohol dehydrogenase 1 (Prunus persica) | ppa007154 | 8 | 41.88/5.93 | 50.63/6.45 | 293 | 27 | +2.75 |
| 9 | Aldolase superfamily protein (Prunus persica) | ppa007696 | 6 | 38.62/6.92 | 36.58/5.63 | 280 | 20 | +3.04 |
| 10 | d-3-Phosphoglycerate dehydrogenase (Arabidopsis thaliana) | gi|15235282 | 2 | 63.57/6.16 | 48.51/5.04 | 136 | 3 | +2.52 |
| 11 | myo-Inositol-1-phosphate synthase 2 (Prunus persica) | ppa004430 | 7 | 56.59/5.96 | 58.31/5.62 | 117 | 35 | –2.55 |
| 12 | Triosephosphate isomerase, putative (Ricinus communis) | gi|255584863 | 3 | 27.66/5.89 | 31.52//6.34 | 198 | 14 | +2.54 |
| 13 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (Aloe viguieri) | gi|33636009 | 5 | 50.60/6.43 | 22.62/6.42 | 320 | 11 | +5.06 |
| 15 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (Calystegia sepium) | gi|21634023 | 4 | 53.10/6.43 | 17.93/5.84 | 343 | 10 | +0 |
| Protein metabolism | ||||||||
| 16 | Glutamine synthetase (Lithospermum erythrorhizon) | gi|4650846 | 2 | 69.85/9.60 | 44.71/6.50 | 179 | 44 | +2.43 |
| 17 | Elongation factor Tu (ISS) (Ostreococcus tauri) | gi|308804561 | 2 | 45.89/6.00 | 49.58/6.53 | 128 | 6 | +2.65 |
| 18 | Insulinase (Peptidase family M16) protein (Prunus persica) | ppa004554 | 7 | 53.82/5.85 | 58.44/5.57 | 218 | 17 | +2.68 |
| 19 | Eukaryotic aspartyl protease family protein (Prunus persica) | ppa004726 | 8 | 53.66/5.45 | 43.68/4.77 | 155 | 19 | +2.85 |
| 20 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein (Prunus persica) | ppa011112 | 5 | 24.76/6.51 | 29.58/6.43 | 188 | 32 | +3.4 |
| 21 | Kunitz family trypsin and protease inhibitor protein (Prunus persica) | ppa011448 | 4 | 23.07/5.20 | 21.92/5.32 | 249 | 19 | +0 |
| Oxidation-reduction | ||||||||
| 22 | Glutathione peroxidase 6 (Prunus persica) | ppa010771 | 7 | 26.23/9.20 | 19.18/6.50 | 168 | 30 | +2.27 |
| 23 | Manganese superoxide dismutase (Prunus persica) | gi|374671153 | 6 | 26.08/8.57 | 27.78/6.80 | 658 | 29 | +2.60 |
| 24 | Ascorbate peroxidase 1 (Prunus persica) | ppa010413 | 6 | 27.46/5.77 | 29.58/5.32 | 383 | 36 | –2.18 |
| 25 | Ascorbate peroxidase 1 (Prunus persica) | ppa010413 | 6 | 27.46/5.77 | 29.07/5.12 | 315 | 36 | –7.13 |
| 26 | Ascorbate peroxidase 1 (Prunus persica) | ppa010413 | 7 | 27.46/5.77 | 31.91/6.54 | 298 | 39 | +6.04 |
| 27 | Peroxidase superfamily protein (Prunus persica) | ppa007748 | 3 | 39.53/5.16 | 47.57/6.59 | 36 | 12 | +7.67 |
| 28 | Copper/zinc-superoxide dismutase (Prunus persica) | gi|381283804 | 2 | 22.36/6.19 | 18.23/5.49 | 151 | 22 | +2.71 |
| 29 | Polyphenol oxidase (Prunus salicina var. cordata) | gi|331272014 | 4 | 64.91/6.48 | 60.93/5.55 | 204 | 8 | +2.73 |
| 30 | Ascorbate peroxidase 1 (Prunus persica) | ppa010413 | 7 | 27.46/5.77 | 30.52/5.37 | 377 | 39 | –9.38 |
| Cell structure | ||||||||
| 31 | Tubulin α-2 chain (Prunus persica) | ppa005617 | 8 | 50.30/4.96 | 36.86/5.92 | 411 | 20 | +3.58 |
| 32 | Actin 7 (Prunus persica) | ppa007242 | 7 | 41.93/5.31 | 49.42/5.46 | 652 | 30 | +3.63 |
| 33 | Actin-depolymerizing factor (Malus×domestica) | gi|33772153 | 2 | 11.19/8.76 | 18.29/6.61 | 104 | 22 | +2.53 |
| Signalling and transcription | ||||||||
| 35 | Calreticulin 1a (Prunus persica) | ppa006226 | 6 | 48.45/4.40 | 58.35/4.25 | 203 | 15 | –2.66 |
| Unclassified | ||||||||
| 36 | Cyanase (Prunus persica) | ppa012598 | 6 | 18.38/6.22 | 16.73/6.79 | 116 | 45 | +2.68 |
a Numbering corresponds to the 2-DE gel in Fig. 2.
b Accession numbers from the PPA database and the NCBI nr 20120421 database.
c The total number of peptides identified.
d MOWSE score probability (protein score) for the entire protein.
e Sequence coverage.
f Average fold change: spot abundance is expressed as the ratio of intensities of upregulated or downregulated proteins between the water treatment after 0 and 10 d. Fold changes had P values<0.05. In this column, ‘+’ means upregulated, ‘–’ means downregulated, and ‘+0’ means that this protein only appears at 10 d after water treatment.