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. 2013 Oct 11;64(16):5221–5230. doi: 10.1093/jxb/ert315

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© The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Expression of hypoxic-responsive genes in hyperhydric Arabidopsis seedlings. After 7 d growth on 0.7% agar, seedlings were transferred to fresh 0.7% agar or to 0.2% Gelrite. Total RNA was extracted from complete seedlings 10 d after transfer. Relative expression levels of the indicated hypoxia-target genes were quantified by qRT-PCR and normalized to Act2 levels. As controls, expression levels of the housekeeping genes UBQ10 and TUB6 were assessed. Each value is the mean ±SE of three biological and six technical replicates, and values are presented as expression fold change (Gelrite versus agar).