DIC microscopy images of the substrates (A-C) and representative optical microscope images of NIH3T3 fibroblast cells cultured on the substrates at 37 °C for 24 hr (D-F) and after lowering the temperature to 25 °C (G-I). Plain PS substrate (A, D, G) and substrates coated with PNIPAM microgels at constant speed of 90 μm/min (B, E, H) and 50.9 mm/min (C, F, I) were compared.