. Author manuscript; available in PMC: 2014 Oct 1.

Published in final edited form as: Langmuir. 2013 Sep 17;29(39):10.1021/la400971g. doi: 10.1021/la400971g

Table 1.

Physical characteristics of different polystyrene substrates with or without PNIPAM microgels and the corresponding cell attachment.

substrate	contact angle measurement(degree)^c,^f		cell adhesion(cells/cm²)^d,^f	cell detachment ^e
substrate	25 °C	37 °C	cell adhesion(cells/cm²)^d,^f	cell detachment ^e
plain PS	77.6 ± 1.9	69.6 ± 1.1	(5.1 ± 4.3) × 10 ³	No
densely-packed PNIPAM microgels on PS ^a	39.6 ± 3.7	41.6 ± 3.9!	(6.6 ± 4.5) × 10 ³	Yes
sparsely-distributed PNIPAM microgels on PS ^b	60.3 ± 2.8	61.6 ± 1.4	(20.7 ± 11.4) × 10 ³^*	Yes
50 μm stripes/50 μm spacings	45.5 ± 3.9	42.8± 4.0	(29.2 ± 8.0) × 10 ³^*	Yes
50 μm stripes/100 μm spacings	38.8 ± 2.7	41.7 ± 3.0	(12.9 ± 5.0) × 10 ³^*	Yes
100 μm stripes/100 μm spacings	43.7 ±1.1	39.7 ± 1.6	(15.4 ± 3.2) ×10 ³^*	Yes!!

PS substrate dip coated with PNIPAM microgels at the withdrawal rate of 90 μm/min.

PS substrate dip coated with PNIPAM microgels at the withdwaral rate of 50.9 mm/min.

Static water contact angle using the captive bubble method. Measurements were conducted at ambient temperature (24.5 °C) or 37 °C by heating the chamber with a heating strip.

Cells were seeded on samples at 12000 cells/cm² and incubated at 37 °C for 24 hours. These substrates were imaged using DIC and cell adhesion per unit area was calculated from at least 10 different optical micrographs using 3 different substrates per condition.

Cell detachment was performed by washing the substrate with tissue culture media at 25 °C.

Data represents mean ± st dev.

p<0.05 vs plain PS.