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Quantifying Interactions of Proline with Bovine Serum Albumin (BSA) by Osmometry. Excess osmolalities ∆Osm of three component solutions of BSA and proline, determined by VPO, are plotted as a function of m2m3, the product of the molal concentrations of BSA and proline. A linear least-squares regression line is shown; from the slope µ23/RT = 20.2 ± 0.6 m−1 (Eq. 2).
