Figure 4.

Grin2b/Nr2b and Bdnf DNA methylation levels in the hippocampus during kainic acid-induced SE. A1) Grin2b/Nr2b DNA methylation was significantly increased in hippocampal area CA1 of the hippocampus during SE compared to controls. No significant change in Grin2b/Nr2b DNA methylation levels were found in area CA3 and the DG region of the hippocampus during SE relative to controls (CA1, t₍₆₎ = 2.47, p = 0.04, n = 7; CA3, t₍₆₎ = 0.77, p = 0.47, n = 7; DG, t₍₆₎ = 0.57, p = 0.59, n = 7; student paired t-test, *p<0.05). A2) DNA Methylation analysis of 13 CpG dinucleotides at the Grin2b/Nr2b gene show significant increases in specific Grin2b/Nr2b DNA methylation at CpG sites in area CA1 during SE compared to controls (Student unpaired t-test, *p<0.05, **p<0.01, n=7-8). B1) No significant change in Bdnf DNA methylation levels were found in areas CA1 and CA3 and the DG region of the hippocampus during SE relative to control animals (CA1, t₍₆₎ = 0.98, p = 0.04, n = 7; CA3, t₍₆₎ = 1.77, p = 0.13, n = 7; DG, t₍₆₎ = 0.78, p = 0.46, n = 7; student paired t-test, p>0.05). B2) No significant changes were found at the Bdnf CpG sites assessed in area CA1 of the hippocampus during SE compared to controls (Student unpaired t-test, p>0.05, n=7-8). Student unpaired t-test, *p<0.05, **p<0.01. Error bars are SEM.