FIG. 2.

Northern blot analysis of LH1 with the ³²P-labeled gene-specific Fg03_09a11 probe hybridized with total RNA isolated from F. graminearum grown under the following conditions: lane 1, infected wheat heads; lane 2, stripped wheat leaves in liquid culture; lane 3, solid rich medium; lane 4, trichothecene-producing liquid culture; lane 5, solid cornmeal substrate. The blot was exposed to film for 5 h at −70°C. The corresponding ethidium bromide-stained gel (showing strong 25S and 18S rRNA bands) prior to blotting is shown under the hybridization panel. Sizes of markers are indicated in kilobases on the left.