TABLE 1.
DNA primers used in this study
| Primer | Sequence 5′→3′ | Gene |
|---|---|---|
| 247 | GGTCAACATGATGTCAGG | hyg |
| 719 | GACGTTGTAAAACGACGGCCAGTG | Plasmid |
| 1113 | ATGTTTCAATATTCCCTATGGC | FgTri11 |
| 1114 | TCATCTTGGGTCAAGGTAC | FgTri11 |
| 1199 | CGGATCATCTATCCTTGAG | FgTri11 |
| 1207 | GGAGGGCCTAGTTTAGG | FgTri11 |
| 1211 | CGTGCCCTGGGGACCAACAC | hyg |
| 1212 | CACAGCCGTGGTTGGTGGGG | hyg |
| 1275 | CAACCATCAAGATGAGCGC | FgTri3 |
| 1276 | CCTCGAGAAACCTATGTACC | FgTri3 |
| 1277 | GTTCTAATAACACAGAGC | FgTri3 |
| 1278 | GTCCTTGGAGTACCACCG | FgTri3 |
| 1328 | CACACAGGAAACAGCTATGACC | Plasmid |
| 1457 | GTCATGGCTCTCATCACCAG | FgLH1 |
| 1459 | GCCTCCCACTCTGGCCGATG | FgLH1 |
| 1462 | GTAGCAGTGCGTCTCTGAG | FgLH1 |
| 1464 | CATGGCGCGCCCAGGGCAGGCGTGTTCTCa | FgLH1 |
| 1466 | GTCAACAAGTGGCGAGATC | FgLH1 |
a
The underlined sequence is an AscI site.