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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Matrix Biol. 2013 Jun 4;32(0):10.1016/j.matbio.2013.05.004. doi: 10.1016/j.matbio.2013.05.004

Figure 5. Spatial expression pattern of amelogenin mRNA by in situ hybridization (ISH) in P0 molars.

Figure 5

(A) LRAP mRNA was not detected in WT tissue. (B) In the TgLRAP mice the LRAP transgene probably as well as endogenous LRAP mRNA was localized in the odontoblasts adjacent to pre-ameloblasts, and in the pre-secretory ameloblasts. (C and E) ISH with a full-length mouse amelogenin (AMG) probe showed abundant expression in pre-ameloblasts and pre-secretory ameloblasts in WT molar. Higher magnification of boxed area (E) showed amelogenin mRNA was detected only in the pre-secretory ameloblasts with the red dashed line indicating the future enamel-dentin junction between the ameloblasts and odontoblasts which have yet to form matrix. (D and F) In the TgLRAP mice AMG mRNA was abundantly expressed in pre-ameloblasts and pre-secretory ameloblasts, as well as in adjacent odontoblasts. Am, ameloblast; Od, odontoblast. Scale bars 100 μm.