Table 2.
Gene | Primer Pair |
---|---|
Amelogenin Exon 2–7 | F: 5′GCTATGCCCCTACCACCTC 3′ R: 5′ GTCCACTTCTTCCCGCTTG 3′ |
Amelogenin Exon 2–9 | F: 5′AACCATCAAGAAATGGGGACC 3′ R: 5′ ACTACATGCCATTGTGTTCTG 3′ |
Full length Amelogenin (for qPCR) | F: 5′CAGCAACCAATGATGCCAGTTCCT 3′ R: 5′ ACTTCTTCCCGCTTGGTCTTGTCT 3′ |
Ameloblastin | F: 5′ CTGTTACCAAAGGCCCTGAA 3′ R: 5′ GCCATTTGTGAAAGGAGAGC 3′ |
Amelotin | F: 5′ ATCAGCCCAGTCATTACCAAAG 3′ R: 5′ AGGTCTGACCCCAGAGTGAG 3′ |
Enamelin | F: 5′ GCTTTGGCTCCAATTCAAAA3′ R: 5′ AGGACTTTCAGTGGGTGT 3′ |
MMP20 | F: 5′ CTCGTCCTTTGATGCAGTGA 3′ R: 5′ TGGACATTAGCTGGGGAAAG 3′ |
Gapdh | F: 5′ GGA CGC ATT GGT CGT CTG G 3′ R: 5′ TTT GCA CTG GTA CGT GTT GAT 3 |