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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Chem Res Toxicol. 2013 Jun 13;26(7):1108–1114. doi: 10.1021/tx4001495

Table 1.

Base substitutions, deletions, and insertions at the sites of MeFapy-dG or the neighboring nucleotides.

Sequence Plasmid
tested,
number		 G to T,
number
(%)		 G to C,
number
(%)		 G to A,
number
(%)		 Deletion,
number
(%)		 Insertion,
number
(%)		 Total
,
%
1 GCTAGCXAGTCC 205 13 (6.3) 4 (2.0) 1 (0.5) - - 8.8
2 GCTAGCXGGTCC 172 144 (8.1) 6 (3.5) 6 (3.5) 3 (1.7) - 16.8
3 GCTAGAXGGTCC 209 95 (4.3) 1 (0.5) 4 (1.9) 1 (0.5) - 7.2
4 GCTAGGXGGTCC 194 10 (5.2) 1 (0.5) 4 (2.1) 14 (7.2) 2 (1.0) 16.0
5 GCTAGTXGGTCC 231 12 (5.2) 1 (0.4) - 14 (6.1) - 11.7
6 GCTAATXGGTCC 244 12 (4.9) 1 (0.4) 121 (5.3) 6 (2.5) - 13.1
7 GCTAGTXTGTCC 238 23 (9.7) 4 (1.7) 3 (1.3) 7 (2.9) - 15.6
8 GCTAGTXAGTCC 229 272 (11.8) 2 (0.9) 4 (1.7) 2 (0.9) - 15.3
9 GCTAGTXCGTCC 215 293 (13.5) 3 (1.4) 12 (5.6) 2 (0.9) - 21.4
1

2 out of 14 G to A transitions were accompanied by a G to C transversion at the 3'-neighboring site (TXG → TAC);

2

2 out of 27 G to T transversions were accompanied by a G to T transversion at the site two nucleotides downstream of the adduct (GTXA → TTTA);

3

1 out of 29 G to T transversions was accompanied by deletion at the site two nucleotides upstream of the adduct (TXCGT → TTC-T);

4

2 out of 14 G to T transversions were accompanied by an additional mutation: G to T transversion at the 3'-neighboring site (CXG → CTT) or a tandem base substitution downstream of the adduct (GCXG → AGTG);

5

1 out of 9 G to T transversions was accompanied by a G to T transversion at the 3'-neighboring site (AXG → ATT).