. Author manuscript; available in PMC: 2014 Mar 13.

Published in final edited form as: J Agric Food Chem. 2013 Feb 27;61(10):2426–2433. doi: 10.1021/jf4004048

Table 3.

Matrix Effect Measured as % Color Reduction and IC₅₀ Values

	8 times dilution		20 times dilution		50 times dilution

Samples	% color reduction	IC₅₀ value (ppb)	% color reduction	IC₅₀ value (ppb)	% color reduction	IC₅₀ value (ppb)
Peanut	47.9%	0.481	47.4%	0.515	35.3%	0.515
Rice	74.4%	0.393	60.1%	0.42	31.7%	0.418
Corn	75.1%	0.5	71.6%	0.444	58.3%	0.46

For the standard assay, 10% methanol-PBS was used. The sample extract was diluted 1 in 7 with PBS (the final methanol concentration in the test solution is 10%), 1 in 19 with 10% Methanol-PBS (the final concentration of methanol in the extract is 13.5% Methanol-PBS) and 1 in 49 with 10% methanol-PBS (final concentration of methonal is 11.4%) for matrix assay. % color reduction was calculated as (1-A_sample/A_methanol)×100, where A_sample is the absorbance (450 nm) of test sample extract without aflatoxin and A_methanol is the absorbance of 10% methanol without aflatoxin.