Skip to main content
. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2013 Jun 11;65:10.1016/j.freeradbiomed.2013.06.010. doi: 10.1016/j.freeradbiomed.2013.06.010

Fig. 3.

Fig. 3

Oxidative stress regulates 4-ClBQ induced toxicity. (A) Cell cultures were incubated with media containing 5.0 mM NAC 5 h prior or 5 h after the addition of 3.0 μM 4-ClBQ. Cell survival was determined by performing a clonogenic assay. Asterisks represent statistical significance compared to untreated cells; # represents statistical significance compared to 3.0 μM 4-ClBQ treated cells; P < 0.05; n = 3. (B) HaCaT cells were infected with adenoviruses AdEmpty (100 MOI), AdMnSOD (50 MOI), AdCAT (50 MOI) or AdMnSOD and AdCAT (50 MOI each). Transgene expression was measured using an immunoblotting assay. Cells were treated with 1.0 μM 4-ClBQ and survival was measured using a clonogenic assay. Surviving fraction was calculated relative to untreated AdEmpty infected cells. Asterisks represent statistical significance compared to AdEmpty infected untreated cells; # represents statistical significance compared to 1.0 μM 4-ClBQ treated AdEmpty infected cells; P < 0.05, n = 3.