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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2013 Jun 11;65:10.1016/j.freeradbiomed.2013.06.010. doi: 10.1016/j.freeradbiomed.2013.06.010

Fig. 5.

Fig. 5

Sodium-selenite supplementation inhibits 4-ClBQ mediated down-regulation of sepp1 expression and protects HaCaT cells from 4-ClBQ induced toxicity. HaCaT cells were cultured in media supplemented with 100 nM of sodium selenite and then treated with 1.0 μM of 4-ClBQ for 24 h. (A) sepp1 mRNA levels were analyzed by performing a quantitative RT-PCR assay. Asterisks represent statistical significance compared to untreated cells; # represents statistical significance compared to 1.0 μM 4-ClBQ treated cells; P < 0.05; n = 3. (B) An immunoblotting assay was used to measure SEPP1 protein levels. A Coomassie blue stained polypeptide band was used for loading correction. (C) A clonogenic assay was used to measure cell survival. Asterisks represent statistical significance compared to untreated cells; # represents statistical significance compared to 1.0 μM 4-ClBQ treated cells; P < 0.05; n = 3.