Figure 5.
Fibronectin accumulation, monocyte/macrophage infiltration and myofibroblast expression at early stage of fibrosis at day 5 after UUO in wild type mice. (A) Fibronectin (FN), one of the major extracellular matrices, was significantly increased by thymosin β4 plus POP inhibitor (Tβ4+POPi) but was significantly decreased by Ac-SDKP. Neither thymosin β4 (Tβ4) nor POP inhibitor (POPi) changed fibronectin accumulation. (B) F4/80 positive infiltrating cells were significantly decreased by Ac-SDKP, but not by other treatments. (C) α-SMA-positive myofibroblasts, a key contributor to extracellular matrix, tended to be increased by thymosin β4 plus POP inhibitor (Tβ4+POPi), and were significantly decreased by Ac-SDKP (×200, immunostaining as indicated).
