Fig. 4.

Sense RNA foci are present in the major glial subtypes. a RNA FISH for sense foci (red) was combined with immunostaining for astrocytes (GFAP), microglia (Iba1) or oligodendrocytes (CAII) (all in green), and DAPI (blue) in the frontal cortex of 8 C9FTLD cases. Scale bar represents 2 μm. The percentage of each cell type containing foci and the number of foci per cell are quantified in (b) and (c), respectively. d Graphical representation of the localisation of sense RNA foci in each cell type. In b, c, each dot represents an individual C9FTLD case with the homozygous C9FTLD case shown in red, and the average and SEM of heterozygous cases shown as long and short horizontal bars, respectively. Significance was determined using the one-way ANOVA and post hoc Bonferroni test (b, c): *p < 0.05, ***p < 0.001, ****p < 0.0001