Fig. 7.

Comparison of RNA foci, p62 and TDP-43 inclusions in C9FTLD patient brain. RNA FISH for either sense or antisense foci was combined with immunostaining for p62 or phospho TDP-43 in the frontal cortex (FC), hippocampus (Hc) and cerebellum (Cb) of 3 heterozygous C9FTLD cases. a Representative images from the frontal cortex show that both sense and antisense foci occur in the same neurons as either p62-positive (TDP-43 negative) or TDP-43 inclusion pathology (both shown in white). b Quantification of co-occurrence of sense or antisense foci with p62 or TDP-43 pathology within the same cell in the frontal cortex, hippocampus and cerebellum shows no obvious associations between pathologies when compared to relative frequencies of each inclusion pathology in the total cell population in respective brain regions (c). d Representative images show that cytoplasmic sense foci (arrowed) can occasionally be found to co-localise within both p62 and TDP-43 inclusions in the hippocampus. Insets show enlarged region containing arrowed foci for each image without channel for p62 or TDP-43 to show clearly that foci are outside the nucleus. e Quantification of the co-localisation of cytoplasmic sense or antisense foci with either p62 or TDP-43 inclusions in the frontal cortex and hippocampus. Scale bars represent 2 μm. Data in graphs is shown as the average and SEM