Table 2.
Immunohistochemical analysis of HIF-2α, TWIST, and CXCR4 expression in 129 PTCs, 61 nodular hyperplasia, and 118 normal thyroid tissue specimens according to the scoring system.
| Score | HIF-2α | TWIST | CXCR4 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Normal thyroid tissue n (%) |
Nodular hyperplasia n (%) |
PTC n (%) |
Normal thyroid tissue n (%) |
Nodular hyperplasia n (%) |
PTC n (%) |
Normal thyroid tissue n (%) |
Nodular hyperplasia n (%) |
PTC n (%) |
|
| 0 | |||||||||
| Negative | 94 (79.7) | 38 (62.3) | 4 (3.1) | 97 (82.2) | 40 (65.6) | 3 (2.3) | 91 (77.1) | 36 (59.0) | 3 (2.3) |
| + | |||||||||
| 1 | 21 (17.8) | 18 (29.5) | 7 (5.4) | 19 (16.1) | 16 (26.2) | 12 (9.3) | 23 (19.5) | 19 (31.1) | 8 (6.2) |
| 2 | 3 (2.5) | 4 (6.6) | 16 (12.4) | 2 (1.7) | 5 (8.2) | 17 (13.2) | 4 (3.4) | 4 (6.6) | 13 (10.1) |
| 3 | 0 (0) | 1 (1.6) | 18 (14.0) | 0 (0) | 0 (0) | 19 (14.7) | 0 (0) | 2 (3.3) | 16 (12.4) |
| 4 | 0 (0) | 0 (0) | 20 (15.5) | 0 (0) | 0 (0) | 22 (17.1) | 0 (0) | 0 (0) | 18 (13.9) |
| ++ | |||||||||
| 6 | 0 (0) | 0 (0) | 16 (12.4) | 0 (0) | 0 (0) | 19 (14.7) | 0 (0) | 0 (0) | 19 (14.7) |
| 8 | 0 (0) | 0 (0) | 23 (17.8) | 0 (0) | 0 (0) | 17 (13.2) | 0 (0) | 0 (0) | 17 (13.2) |
| +++ | |||||||||
| 9 | 0 (0) | 0 (0) | 16 (12.4) | 0 (0) | 0 (0) | 17 (13.2) | 0 (0) | 0 (0) | 20 (15.5) |
| 12 | 0 (0) | 0 (0) | 9 (7.0) | 0 (0) | 0 (0) | 3 (2.3) | 0 (0) | 0 (0) | 15 (11.6) |
The immunohistochemical scores in PTCs, nodular hyperplasia, and normal thyroid tissue specimens were determined as the multiplication of proportion score and intensity score.