Table 1.
Primers used for cloning N gene domains of CHPV.
| S. no. | Construct | Oligo name | Primer sequence |
|---|---|---|---|
| 1 | BD-N1 and AD-N1 | N1 F NdeI (BD/AD) | GGAAGTGACATATGAGTTCTCAAGTATTCTGC |
| N1 R BamHI (BD/AD) | GCTAACAGGATCCGAAGAATGCCCCTGGAAAC | ||
| 2 | BD-N2 and AD-N2 | N2 F NdeI (BD/AD) | GGAAGTGACATATGGAAGACCCAGTGGAGTTTC |
| N2 R BamHI (BD/AD) | GCTAACAGGATCCATGGAAACTGGGATTTTTTGTTG | ||
| 3 | BD-N3 and AD-N3 | N3 F NdeI (BD/AD) | GGAAGTGACATATGACTCTGTCACACCTCCAG |
| N3 R BamHI (BD/AD) | GCTAACAGGATCCTCATGCAAAGAGTTTCCTGGC | ||
| 4 | GST-N1 | N1 F BamHI (pGEX-4T3) | GCTAACAGGATCCATGAGTTCTCAAGTATTCTGC |
| N1 R XhoI (pGEX-4T3) | GCTAACACTCGAGGAAGAATGCCCCTGGAAAC | ||
| 5 | GST-N2 | N2 F BamHI (pGEX-4T3) | GCTAACAGGATCCATGGAAGACCCAGTGGAGTTTC |
| N2 R XhoI (pGEX-4T3) | GCTAACACTCGAGATGGAAACTGGGATTTTTTGTTG | ||
| 6 | GST-N3 | N3 F BamHI (pGEX-4T3) | GCTAACAGGATCCATGACTCTGTCACACCTCCAG |
| N3 R XhoI (pGEX-4T3) | GCTAACACTCGAGTCATGCAAAGAGTTTCCTGGC |
Primers used for PCR amplification of Chandipura Virus N1, N2, and N3 domains of nucleocapsid gene (F: forward primer and R: reverse primer). The names of the restriction enzymes are in italics and their recognition sequences in bold.