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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: J Am Chem Soc. 2013 Jun 27;135(27):10.1021/ja404404h. doi: 10.1021/ja404404h

Figure 1.

Figure 1

The effect of PEG on the ATPase activity of eIF4A. a) The influence of the size of PEG on the ATPase activity. eIF4A at 3 μM was incubated for 1h with 3 μM poly(U) (black) and without RNA (grey), 5 mM ATP with 0.1 mCi of [α-32P] ATP and 4% PEG of varying sizes. The amount of released Pi was determined using the Norit assay. b) Ratio of Pi:ATP after incubation of 3 μM eIF4A at varying concentrations of PEG 8k. Amounts of Pi and ATP in the samples (same content as described in a) containing varying concentrations of PEG 8k were determined using a TLC assay. c) Dependence of the ATPase activity for increasing amounts of ATP with and without 8% PEG 8k. Sample content was the same as described in A) with the exception of varying ATP concentrations. All data points are averages of duplicates.