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. Author manuscript; available in PMC: 2013 Nov 17.
Published in final edited form as: Bioorg Med Chem. 2007 Aug 24;15(23):10.1016/j.bmc.2007.08.029. doi: 10.1016/j.bmc.2007.08.029

Figure 5.

Figure 5

Dependence of the rate constant for slow inhibition of Tc-SAHH and Hs-SAHH on the concentration of ribavirin. Tc-SAHH and Hs-SAHH (9.07 μM, reconstituted with NAD+) were incubated with various concentrations of ribavirin (0-1.8 mM) and 50 μM NAD+ in 50 mM phosphate buffer (pH 7.2), 1 mM EDTA at 37 °C. The initial velocity of the development of NADH was measured by use of fluorescence (excitation at 340 nm and emission at 450 nm). Data were fit to the hyperbolic saturation model (eq 4) and results are shown in Table 1.