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. 2013 Oct 25;47(5):458–465. doi: 10.4132/KoreanJPathol.2013.47.5.458

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© 2013 The Korean Society of Pathologists/The Korean Society for Cytopathology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1 — Detecting immunoglobulin (IG) and T cell receptor (TCR) gene rearrangement by 4% Metaphor agarose gel (A) and 8% non-denaturing polyacylamide gel (B) using the BIOMED-2 protocols. Results of IG/TCR gene clonality detection are indicated by the presence or absence of a PCR product of the expected size. All reactions on non-denaturing polyacrylamide gels and Metaphor agarose gels revealed the same results. The case of the photos showed positivity for TCRB_A and TCRB_C on both gels. The expected size of the PCR products were as follows: IGH_A 310-360 bp; IGH_B 250-295 bp; IGH_C 100-170 bp; IGH_D 110-290, 390-420 bp; IGH_E 100-130 bp; IGK_A 120-160, 190-210, 260-300 bp; IGK_B 201-250, 270-300, 350-390 bp; TCRB_A 240-285 bp; TCRB_B 240-285 bp; TCRB_C 170-210, 285-325 bp; TCRG_A 145-255; TCRG_B 80-220 bp. M, DNA size marker; PC, positive control; NC, negative control.