Fig. 2.
Lipid-insertion enables modification of RBC-NPs with FITC. (A) Flow cytometry histograms of plain RBC ghosts (black) and RBC ghosts incorporated with FITC–linker–lipid (green). (B) Fluorescence microscopy visualization of RBC ghosts modified with FITC (green). Scale bar = 8 µm. (C) FITC–linker–lipid was incubated with RBC ghosts derived from 1 mL of mouse blood. The amount of FITC–linker–lipid incorporated onto the RBC ghosts was then quantified after 30 min of incubation and plotted against the initial input. (D) Physicochemical characterizations (size and zeta potential) of both FITC-modified and unmodified RBC-NPs. (E) SEM images of FITC-modified and unmodified RBC-NPs. Insets represent a single particle with a size of ~80 nm. Scale bars = 500 nm. (F) Colocalization of the polymeric core (red) and the FITC-modified RBC membrane shell (green) upon intracellular uptake by KB cells. Cellular nuclei were stained with DAPI (blue). Scale bars = 8 µm.