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. Author manuscript; available in PMC: 2013 Nov 18.
Published in final edited form as: Nanoscale. 2013 Aug 1;5(19):10.1039/c3nr03064d. doi: 10.1039/c3nr03064d

Fig. 4.

Fig. 4

Lipid-insertion enables targeting functionalization of RBC-NPs with AS1411 aptamer. (A) Schematic representation of AS1411–linker–lipid. (B) Flow cytometry histograms of MCF-7 cells alone (black) and the cells incubated with AS1411-functionalized RBC-NPs (red), non-targeted RBC-NPs (blue), and AS1411-functionalized RBC-NPs together with the free AS1411 aptamer (orange). (C) Quantification of the mean fluorescence intensity of the histograms in (B). (D) Fluorescence microscopy of MCF-7 cells incubated with AS1411-functionalized RBC-NPs, non-targeted RBC-NPs, and AS1411-functionalized RBC-NPs together with free AS1411. A fluorescent probe DiD was loaded inside the RBC-NPs for visualization (red) and cellular nuclei were stained with DAPI (blue). Scale bars = 25 µm.