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. Author manuscript; available in PMC: 2013 Nov 18.
Published in final edited form as: J Am Chem Soc. 2013 Aug 28;135(36):10.1021/ja404653q. doi: 10.1021/ja404653q

Figure 5.

Figure 5

Extending the dynamic range of metal binding switches using a mixture of an allosteric activator and an allosteric inhibitor. While the switch in the presence of a single allosteric effector shows a limited dynamic range (only 8-fold in target concentration; see dashed curves), this same dynamic range is widened to almost 3 orders of magnitude in the presence of the activator/inhibitor mixture (solid line). Here the switch concentration was held at 10 nM and the activator (21-base) and inhibitor (20-base) concentrations at 7 and 3 nM respectively (see SI for details).