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. 2013 Sep 17;21(11):2043–2053. doi: 10.1038/mt.2013.187

Figure 5.

Figure 5

Triptolide (TPL) enhanced vesicular stomatitis virus (VSV) apoptosis in PC3 cell line through caspase-3 activation and a decrease in Bcl-2 expression. PC3 cells were pretreated with or without TPL and then infected with VSV-Δ51-GFP as described above. The level of apoptosis was measured by flow cytometry using (a) cleaved caspase-3-PE (CC3) and (d and e) Bcl-2-V450 antibodies. The levels of annexin-V binding and cleaved caspase-3 were also measured at day 1 and day 2 postinfection in VSV-infected PC3 pretreated or untreated with TPL (6.25 nmol/l) and/or with ZVAD (100 μmol/l; b and c). Histograms shown are representative of at least three independent experiments and the values represent the means ± SEM. P values were calculated to compare the following: Each value for TPL plus VSV was compared with vehicle control. *P ≤ 0.05; **P ≤ 0.01. TPL alone was compared with NI. NI denotes non–TPL-treated and non–VSV-infected samples. §P ≤ 0.05; §§P ≤ 0.01. VSV-infected cells were compared with NI. ††P ≤ 0.01. VSV plus TPL value was compared with VSV plus TPL plus ZVAD. ‡P ≤ 0.05; ‡‡P ≤ 0.01.