Figure 7.

Necrosulfonamide and virus replication after necrosis inhibition. (a) Effect of inhibiting MLKL on Lister-dTK induced cell death. Cells were treated with necrosulfonamide (MLKL inhibitor) following infection with virus, and cell survival determined by MTT assay 72 hours postinfection. (b) A2780CP and TOV21G cells were infected with Lister-dTK at MOI 100. Infectivity was assessed by RFP flow cytometry 12 hours postinfection (left). Cell survival was assessed 24 hours postinfection in the presence and absence of 100 µmol/l necrostatin-1 or 10 µmol/l necrosulfonamide.