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. 2013 Oct 1;21(11):1996–2007. doi: 10.1038/mt.2013.196

Figure 2.

Figure 2

EJ1 peptide induces the formation of inactive ERBB multimers. (a, c) MDA-MB-468 cells or (b) BxPC3 cells were treated with 100 or 50 ng/ml EGF (respectively) in combination with water (Veh), 20 μmol/l CP, or 20 μmol/l EJ1 for the indicated times and lysed. Protein levels were determined as indicated. (d) MDA-MB-468 cells were treated with peptides for 10 minutes on ice. Then, 100 ng/ml EGF was added to the medium and incubated for another 10 minutes on ice, followed by cross-linking with 3 μmol/l DMS. Lysates were evaluated by western blotting for ERBB1 and β-actin. (e) MDA-MB-468 cells were treated similarly, but without cross-linker, lysed, and immunoprecipitated (ERBB1 Ab-13). Molecular weights are shown to the right.