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Kinetic constants of PH and ToMO on benzene and phenola
| Substrate | PH
|
ToMO
|
||||
|---|---|---|---|---|---|---|
| kcat (s−1) | Km (μM) | kcat/Km (103 s−1 μM−1) | kcat (s−1) | Km (μM) | kcat/Km (103 s−1 μM−1) | |
| Benzene (cells) | 0.092 | 25 | 3.7 | 0.36 | ∼0.2 | 1,810 |
| Phenol (cells) | 1.02 | 0.6 | 1,696 | 1.00 | 2.18 | 460 |
| Phenol (complex) | 0.286 | 0.61 | 469 | |||
Constants were measured on cells (benzene and phenol) or on the reconstituted complex (phenol). Errors were always lower than 6% except those for Km on benzene, which was about 25%.
