Table 2. BRCA1 and BRCA2 markers, primers and labels for universal PGD tests.

Protocol	Marker	Primer sequence (5′→3′)	Tag	Size (bp)
BRCA1 (six markers)
	D17S932	Fw—ACACGGATGGCCTTTTAGAAAGTGGTC Rev—AACACACAGACTTGTCCTACTGCCAT	VIC	145–157
	D17S1814	Fw—ATGCTCCCCAATGACGGTGATG Rev—AGCTGGAGGTTGGCTTGTGGAT	NED	150–166
	D17S950	Fw—CATACACAGCACTTGCCCCCATGT Rev—ACAACAGCACAACGCCCTGCAC	FAM	169–187
	D17S1787	Fw—TGCAAGACCCTTCACGCTTTGTC Rev—CTTGGTGGTTCCCTTCGTCCTTG	NED	186–198
	BRCA1_dist24AC	Fw—TGCAGAACAATTGTAGCAGCACACAG Rev—GTGGTCAGAACAATGCAAATTGAAGC	VIC	205–235
	D17S800	Fw—ACATCACCCAGGGAGGTGAGTTC Rev—AAGTGGGAGGAGCCATGAATGA	NED	266–276
BRCA2 (eight markers)
	D13S1695	Fw—TGTTCTAATGCCTGGGTATCATCC Rev—CAGGTGATCTGAGACTCAATAGCTTAACA	VIC	98–122
	D13S267	Fw—TCCTCCCCATCCACCTTTCTCC Rev—CAGGTCCCACCATAAGCACAAGC	FAM	133–147
	D13S171	Fw—AAGGGAAGGAGAAAGGGGAGGTG Rev—GCATTGACCTTAGGGCCATCCA	NED	150–166
	D13S289	Fw—GGTTGAGCGGCATTGAAAACAG Rev—CACCTTCATCACCACCTTGATATGG	FAM	163–177
	BRCA2_dist18AC	Fw—GCCGCCTTTCACGTAAGCACAG Rev—AATGGGAACCCAATTCAGCAAGG	PET	180–210
	D13S260	Fw—GGATCTGCTTGCAATGCCCAAA Rev—TCTCCCAGATATAAGGACCTGGCTATG	VIC	210–224
	D13S1698	Fw—TGGGATTACAGGCTTGAGCCACA Rev—TCTGACACAGCTGGTTTGTCTATTCACC	FAM	215–235
	BRCA2STR19	Fw—GAATTTGTGTTCCAGGTGAGAATTGC Rev—ATGGGGTGCCTATGGCCTGAA	NED	235–259

Fw=forward, Rev=reverse, Tag=fluorescent dyes tagged to 5′ end of forward primers.