Table 3. Validation of universal protocols.

Gene	Marker	No. of single cells tested	No. of heterozygotes	ADO (%) per locus	PCR efficiency (%) per locus	Positive blanks per locus
BRCA1	D17S1814	57	54	1.8	96.5	0/13
	D17S800	57	55	1.8	96.5	0/13
	D17S1787	57	55	1.8	96.5	0/13
	D17S932	57	55	1.8	96.5	0/13
	BRCA1_dis24AC	57	55	0.0	96.5	1/13
	D17S950	57	54	0.0	96.5	0/13
BRCA2	D13S289	106	104	1.9	98.1	0/24
	D13S260	106	69	5.8	98.1	0/24
	D13S1698	106	104	5.8	98.1	0/24
	BRCA2STR19	106	103	1.9	97.2	0/24
	D13S171	106	35	2.9	98.1	0/24
	D13S1695	106	34	0.0	97.2	1/24
	BRCA2_dist18AC	106	70	0.0	97.2	0/24
	D13S267	106	69	0.0	98.1	0/24