Figure 4.

The imprinting status of the grand-grand-paternal allele after grandmaternal and maternal transmissions. The OCT4 binding site mutation apparently causes incomplete demethylation upon grandmaternal transmission (A), and further methylation upon maternal transmission (B). Of note, further demethylation, as in A, was not seen in B (marked by the X), suggesting that only paternal alleles can be demethylated in the maternal gonads. Blue=methylated (paternal pattern) and red=unmethylated (maternal pattern).