FIGURE 4.

HuR deficiency in Th17 cells impaired IL-17 mRNA stability. CD4⁺ T cells were isolated from spleen of WT control mice and HuR KO mice. Cells were stimulated as described in Fig. 1 in the presence of Th17 cell–polarizing cytokines for 5 d. Th17-polarized cells were untreated or treated with actinomycin D (Act D; 3 μg/ml) and harvested at 1, 3, and 5 h. Levels of IL-17 (A) and Stat-3 (B) mRNA were determined by real-time PCR. Data in (A) were derived from at least three independent experiments. Data in (B) were derived from two independent experiments.