| HuR forward |
5′-ACTGCA GGGATGACATTGGGAGAA-3′ |
| HuR reverse |
5′-AAGCTTTGCAGATTCAACCTCGCC-3′ |
| Stat3 forward |
5′-TAGCCGATTCCTGCAAGAGTCCAA-3′ |
| Stat3 reverse |
5′-CGGGCAATTTCCATTGGCTTCTCA-3′ |
| IFN-γ forward |
5′-ACAGCCACTGCATTCCCAGTTT-3′ |
| HuR forward |
5′-TCTCGGAAGGACTTGCAGACAT-3′ |
| HuR reverse |
5′-TGTGTCTCTGATGCTGTTGCTGCT-3′ |
| IL-17 reverse |
5′-AGGAAGTCCTTGGCCTCAGTGTTT-3′ |
| GM-CSF forward |
5′-TGGAAGCATGTAGAGGCCATCA-3′ |
| GM-CSF reverse |
5′-GCGCCCTTGAGTTTGGTGAAAT-3′ |
| Bcl6 forward |
5′-CATACAGAGATGTGCCTCCATAC-3′ |
| Bcl6 reverse |
5′-CCCATTCTCACAGCTAGA ATC C-3′ |
| Foxp3 forward |
5′-TACACCCAGGAAAGACAGCAACCT-3′ |
| Foxp3 reverse |
5′-TCTGCTTGGCAGTGCTTGAGAA-3′ |
| RORγt forward |
5′-ACAGCCACTGCATTCCCAGTTT-3′ |
| RORγt reverse |
5′-TCTCGGAAGGACTTGCAGACAT-3′ |
| Sequence of Mouse RT-PCR Primers in Biotin Pulldown |
|---|
| IL-17 ORF forward+T7 |
5′-GCT TCT AAT ACG ACT CAC TAT AGG ATG AGT CCA GGG AG-3′ |
| IL-17 ORF reverse |
5′-TTA GGC TGC CTG GCG GAC AA-3′ |
| IL-17 sect1 forward+T7 |
5′-GCT TCT AAT ACG ACT CAC TAT AGG ACA GAG ACC CGC GG-3′ |
| IL-17 sect1 reverse |
5′-GTC TTG AAT TTT GTC TTC TTC AG-3′ |
| IL-17 sect2 forward+T7 |
5′-GCT TCT AAT ACG ACT CAC TAT AGG CCA GAA ATT TTA TT-3′ |
| IL-17 sect2 reverse |
5′-CCA AAA CTT TAT TAT TAC AGT GAT ATT GTT ACA A-3′ |
| IL-17 3′ UTR forward+T7 |
5′-GCT TCT AAT ACG ACT CAC TAT AGG ACA GAG ACC CGC GG-3′ |
| IL-17 3′ UTR reverse |
5′-CCA AAA CTT TAT TAT TAC AGT GAT ATT GTT ACA A-3′ |
| GM-CSF ORF forward+T7 |
5′-GCT TCT AAT ACG ACT CAC TAT AGG ATG TGG CTG CAG AA-3′ |
| GM-CSF ORF reverse |
5′-TCA TTT TTG GCC TGG TTT TTT GCA TTC AAA GGG G-3′ |
| GM-CSF 3′ UTR forward+T7 |
5′-GCT TCT AAT ACG ACT CAC TAT AGG GGA AGC CCA GGC CAG-3′ |
| GM-CSF 3′ UTR reverse |
5′-CTGGTAAGACATTCTCAATAAATAGAGTTGC-3′ |
