Fig 4.

Test results of three pairs of new markers (Y₁, X₁ and XY₁) in 4 phenotypic male and 4 female individuals from Dongting Lake wild population. The 398 bp Y-specific fragment amplified by Y₁ primer pair, the 823bp X-specific fragment by X₁ primer pair, as well as 955 bp X-specific fragment and 826 bp Y-specific fragment by XY₁, are indicated by arrows on the right. The DL2000 DNA marker sizes are shown on the left. YY individual is the control for the YY super-male.