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Test results of the XY1 marker in other four wild populations (8 male and 8 female individuals from each population) from Liangzi Lake (Wuhan), Hong Lake (Honghu) Chang Lake (Jingzhou), and South Lake (Zhongxiang). The 955 bp X-specific fragment and 826 bp Y-specific fragment amplified by XY1 primer pair are indicated by arrows on the right. The DL2000 DNA marker sizes are shown on the left. YY individual is the control for the YY super-male.
