TABLE 2.
Primers
| Primer | 5′ → 3′ sequencea | Site created |
|---|---|---|
| LPD3 | CACTTGATGACTTTCTCGGCAC | |
| LPD6 | CACCGATCTCGTCATCAAACG | |
| LPD8 | TCTTCAACCCACTTTGGGAAG | |
| LPD9 | CACCGATCTCGTCATCAAAGG | |
| LPD16 | CTCGTATTCCCAGCCGTTATC | |
| LPINV2 | ATTCGCCGATAGACAAGTGG | |
| LPREP2 | CTCCGGTAAGGCTTTCAACG | |
| LPΔPDC1 | AGCCTGCAGACCGACACTGATCCACTCAT | PstI |
| LPΔPDC4 | GGAAAGCTTGCAGAGCAAGGTAAG | HindIII |
| LPFP3 | CCAGAATTCACCGATCTCGTCATCAAAGG | EcoRI |
| LPYFMUT1 | CGAAGCTTCGCTTTCCAGCACCGAAG | HindIII |
| LPPADR1 | CCCATGGCGCAAAAAAATAAGTTACAA | NcoI |
| LPPADR2 | CCCCTCGAGCTCCGGTAAGGCTTTCAAC | XbaI |
| LPGS1 | CCCTGCAGTGGAAATTCCTCCTTGACG | PstI |
| LPGS2 | CCGAATTCTAGAATTACCTTCCTTATG | EcoRI |
| LPLDH1 | CCGTGACCGACGGCAGCCGC | |
| LPLDH2 | CCGTCGCCCACTAACACAAC | |
| M13 universal | TGTAAAACGACGGCCAGT |
a
Underlined nucleotides correspond to restriction sites named in last column.