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. 2013 Nov 15;46(Pt 6):1903–1906. doi: 10.1107/S0021889813027581

Table 1. Well components of Hampton screen HR2-130 exhibiting SHG activity.

Well No. Salt Buffer Precipitant SHG activity
3 (A3) None None 0.4 M ammonium phosphate monobasic Strong
15 (B3) 0.2 M ammonium sulfate 0.1 M sodium cacodylatetrihydrate pH 5.6 30% w/v polyethylene glycol 8000 Weak
16 (B4) None 0.1 M HEPES sodium pH 7.5 1.5 M lithium sulfate monohydrate Strong
17 (B5) 0.2 M lithium sulfate monohydrate 0.1 M Tris–HCl pH 8.5 0.1 M Tris–HCl pH 8.5 Strong
20 (B8) 0.2 M ammonium sulfate 0.1 M sodium acetate trihydrate pH 4.6 0.1 M sodium acetate trihydrate pH 4.6 Medium
23 (B11) 0.2 M magnesium chloride hexahydrate 0.1 M HEPES sodium pH 7.5 30% v/v polyethylene glycol 400 Weak
47 (D11) None 0.1 M sodium acetate trihydrate pH 4.6 2.0 M ammonium sulfate Medium
48 (D12) None 0.1 M Tris–HCl pH 8.5 2.0 M ammonium phosphate monobasic Strong
61 (F1) 0.2 M ammonium sulfate 0.1 M sodium acetate trihydrate pH 4.6 30% w/v polyethylene glycol monomethyl ether 2000 Medium
63 (F3) 0.5 M ammonium sulfate 0.1 M sodium citrate tribasic dihydrate pH 5.6 1.0 M lithium sulfate monohydrate Strong
65 (F5) None 0.1 M sodium citrate tribasic dihydrate pH 5.6 35% v/v tert-butanol Weak
73 (G1) 0.01 M cobalt(II) chloride hexahydrate 0.1 M MES monohydrate pH 6.5 1.8 M ammonium sulfate Medium
82 (G10) 0.05 M cadmium sulfate hydrate 0.1 M HEPES pH 7.5 1.0 M sodium acetate trihydrate Weak
84 (G12) None 0.1 M HEPES pH 7.5 4.3 M sodium chloride Weak
89 (H5) 0.01 M nickel(II) chloride hexahydrate 0.1 M Tris pH 8.5 1.0 M lithium sulfate monohydrate Strong